Photooxidation-induced fluorescence amplification system for an ultra-sensitive enzyme-linked immunosorbent assay (ELISA)
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چکیده
منابع مشابه
Sensitive enzyme linked immunosorbent assay (ELISA) for xanthine oxidase.
The enzyme, xanthine oxidase, has been implicated as a major source of destructive free radicals in ischemia-reperfusion injury associated with a range of pathological states [l]. Enzymic activity has been detected in many mammalian tissues [4] by using, variously, spectrophotometric [51, radiometric[6] and fluorimetric [71 assays. Apart from bovine milk fat globule membrane, which has long pro...
متن کاملDEVELOPMENT OF A SIMPLE AND SENSITIVE ENZYME- LINKED IMMUNOSORBENT ASSAY (ELISA) FOR CLINICAL MEASUREMENT OF TESTOSTERONE USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay using a homologous combination of antiserum raised against testosterone-3-0-carboxymethyloxime-bovine serum albumin (T-3-0-CMO-BSA ) and penicillinase-labelled T-3-0-CMO was developed. This assay was utilized to measure testosterone in serum samples of male and female subjects. The sensitivity of the assay is 50pg/well and the antibody developed crossrea...
متن کاملThe ELISA, enzyme-linked immunosorbent assay.
Featured Article: Engvall E, Perlmann P. Enzymelinked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971;8:871– 4. This paper was my first as a graduate student of Professor Peter Perlmann at Stockholm University. At the time, RIAs were in full bloom, but they were too sophisticated for many areas of research and diagnosis because they required expensive e...
متن کاملDevelopment of an enzyme - linked immunosorbent assay ( ELISA ) for the
6 Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of 7 Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania; Veterinary 8 Mycology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, 9 University of Liège, Liège, Belgium; Dermatology and Venereology Department, Laboratory of 10 Mycology, Centr...
متن کاملAn enzyme-linked immunosorbent assay (ELISA) for urinary free cortisol.
Since the introduction of our plasma cortisol enzyme-linked immunosorbent assay (ELISA) [l] using affinity purified antiserum [2] into the routine steroid laboratory, we have embarked on the development of a simple urinary free or unconjugated cortisol ELISA which would allow measurement of both urine and plasma cortisol on the one ELISA plate. This paper describes for the first time an ELISA f...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2021
ISSN: 2045-2322
DOI: 10.1038/s41598-021-85107-7